久久久天堂国产精品女人,国产AV国片精品一区二区,伊人久久大香线蕉av影院,日韩av

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>CRL-9444MeT-5A細(xì)胞, 人類肋膜間皮細(xì)胞

MeT-5A細(xì)胞, 人類肋膜間皮細(xì)胞

簡要描述:MeT-5A細(xì)胞, 人類肋膜間皮細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞,細(xì)胞庫管理規(guī)范,提供 培養(yǎng)條件

  • 產(chǎn)品型號:CRL-9444
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-16
  • 訪  問  量:2170

產(chǎn)品分類

Product Category

詳細(xì)介紹

MeT-5A細(xì)胞, 人類肋膜間皮細(xì)胞

ATCC® Number:CRL-9444™    Price:$329.00
Designations:MeT-5A

Depositors:The United States of America

Biosafety Level:2 [Cells contain polyomavirus DNA sequences ]

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:epithelial


Source:Tissue: mesothelium
Cell Type: epithelialvirus transformed


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
MeT-5A細(xì)胞, 人類肋膜間皮細(xì)胞

Tumorigenic:No

DNA Profile (STR):Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 11,13

D16S539: 12

D5S818: 12

D7S820: 10

THO1: 6,9.3

TPOX: 8

vWA: 15,18



Comments:Mesothelial cells were isolated from pleural fluids obtained from non-cancerous individuals.

The cells were transfected with the pRSV-T plasmid (an SV40 ori- construct containing the SV40 early region and the Rous sarcoma virus long terminal repeat) and cloned.

The cells can be used to screen chemical and biological agents for activity as growth factor, carcinogens, mutagens, etc.

The cells stain positively for vimentin, keratins and SV40 T antigen.



Propagation:ATCC complete growth medium: Medium199 with Earle's BSS, .75 mM L-glutamine and 1.25 g/L sodium bicarbonate supplemented with with 3.3 nM epidermal growth factor (EGF), 400 nM hydrocortisone, 870 nM insulin, 20 mM HEPES, trace elements (see above reference) and 10% fetal bovine serum.
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days


Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Related Products:recommended serum:ATCC 30-2020

References:21937: Reddel RR, et al. Immortalized human bronchial epitherial mesothelial cell lines. US Patent 4,885,238 dated Dec 5 1989
23292: Lechner JF, et al. Asbestos-associated chromosomal changes in human mesothelial cells. Proc. Natl. Acad. Sci. USA 82: 3884-3888, 1985. PubMed: 2987952

























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国产专区| 亚洲国产中文无码视频在线观看| 亚洲精品无码少妇30P| 国产成人精品无码免费看| 国产成人亚洲综合精品| 国产麻豆剧传媒精品国产AV| 亚洲色图第一页| 久久黄色影院| 人人爱我我爱人人是谁说的| 狠狠爱无码一区二区三区| 久久亚洲国产成人影院| 草草影院精品一区二区三区| 国内精品伊人久久久久AV一坑| 欧美精品亚洲精品日韩专区va| 国产在线观看无码免费视频| 久久精品超碰av无码| 冰皮月饼| 黑人hd| 97视频在线| 无码人妻丰满熟妇区BBBBXXXX| 天堂av男人在线播放| 亚洲综合AV色婷婷国产野外| 亚洲成A∧人片在线观看无码| 性色AV 一区二区三区| 51久久| 色狠狠久久av友田真希| 西乌珠穆沁旗| 无码精品尤物一区二区三区| av免费网址在线观看| 国产精品自产拍高潮在线观看| 亚洲AV人无码激艳猛片| 国产精品久久久久精品综合紧| 一本色道久久综合亚洲精品| 无码熟妇人妻在线视频| 97久久精品亚洲中文字幕无码| 色偷偷av男人的天堂| 亚卅国产一级毛片| 亚洲区麻豆砖码区| 国产69久久精品成人看| 九九九精品成人免费视频7| 亚洲日韩一区二区一无码|