久久久天堂国产精品女人,国产AV国片精品一区二区,伊人久久大香线蕉av影院,日韩av

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>CRL-11005FL62891細(xì)胞, 人肝細(xì)胞

FL62891細(xì)胞, 人肝細(xì)胞

簡要描述:FL62891細(xì)胞, 人肝細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-11005
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2025-03-26
  • 訪  問  量:1892

產(chǎn)品分類

Product Category

詳細(xì)介紹

FL62891細(xì)胞, 人肝細(xì)胞

ATCC® Number:CRL-11005™    Price:$353.00
Designations:FL 62891

Depositors:ImClone Systems Inc.

Biosafety Level:2 [Cells contain SV-40 viral DNA sequences ]

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:fibroblast

Source:Organ: liver
Cell Type: immortalized with SV40 large T antigen


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
FL62891細(xì)胞, 人肝細(xì)胞

Age:fetus

Comments:The cell line was derived from human fetal liver tissue, and immortalized by transformation with an ecotropic retrovirus containing a temperature sensitive SV40 T-antigen (tsA58) and a G418 resistance gene. After several days at 37C, the temperature of the medium was lowered to 32C. Cells were selected with G418. The selected cells were expanded and maintained. The cells produce flk-1 and some flk-2 ligands.

Propagation:ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate, and 15% fetal bovine serum
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 32.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 32°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 32°C.


Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days


Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Doubling Time:48 hours

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005

recommended serum:ATCC 30-2020



References:70742: Lemischka IR. Nucleic acids encoding fragments of hematopoietic stem cell receptor flk-2. US Patent 5,270,458 dated Dec 14 1993
70744: Lemischka IR. Tyrosine kinase receptor flk-2 and fragments thereof. US Patent 5,367,057 dated Nov 22 1994
70745: Lemischka IR. Tyrosine kinase receptor human flk-2-specific antibodies. US Patent 5,548,065 dated Aug 20 1996
70746: Lemischka IR. Nucleic acids encoding soluble human FLK-2 extracellular domain. US Patent 5,621,090 dated Apr 15 1997
70747: Lemischka IR. Antibodies against tyrosine kinase receptor flk-1. US Patent 5,747,651 dated May 5 1998
70748: Lemischka IR. Method for isolating stem cells expressing flk-1 receptors. US Patent 5,912,133 dated Jun 15 1999






















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
а∨天堂在线中文免费不卡| 午夜福利视频一区二区手机免费看| 亚洲AV无码成人精品区H| 国产日韩精品欧美2020区| 久久久久逼| 亚洲色无码播放亚洲成av| 精品香蕉一区二区三区| 米奇777超碰欧美日韩亚洲| 国产精品人妻熟女丝袜13p| 亚洲中文一本无码av在线无码| 成人免费毛片内射美女APP| 天天射天天射| 欧美黑人巨大XXXXX| 成人AV在线一区二区三区 | 97久久精品无码一区二区天美| 免费无码无遮挡裸体视频在线观看| 久久久久久久久久久久久久久久久| porny九色| 国产内射999视频一区| 免费看片AV免费大片| 国产成人综合日韩精品无码| 精品无码久久久久久午夜福利| 天干夜天干天天天爽视频| 日韩av自拍| h片在线免费观看| 四虎精品在线成人影院| 熟妇人妻VA精品中文字幕| 国产无人区卡一卡二扰乱码| 久久久久人妻精品一区二区三区 | 懂色av懂色aⅴ精彩av| 久久亚洲AV成人无码| 亚洲AV无码成人精品区狼人影院| 亚洲精品无码专区| 久久精品国产精品亚洲下载| 秋霞鲁丝无码一区二区三区| 四虎成人精品永久网站 | 国产精品内射久久久久欢欢| 高潮潮喷奶水飞溅视频无码| 中文字幕亚洲综合久久综合| 国产真人无码作爱视频免费 | 蜜臀av性久久久久蜜臀aⅴ麻豆|