久久久天堂国产精品女人,国产AV国片精品一区二区,伊人久久大香线蕉av影院,日韩av

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>HTB-1J82 細(xì)胞,人膀胱移行細(xì)胞癌細(xì)胞

J82 細(xì)胞,人膀胱移行細(xì)胞癌細(xì)胞

簡要描述:J82 細(xì)胞,人膀胱移行細(xì)胞癌細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):HTB-1
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問  量:1414

產(chǎn)品分類

Product Category

相關(guān)文章

Related Articles

詳細(xì)介紹

J82 細(xì)胞,人膀胱移行細(xì)胞癌細(xì)胞

ATCC® Number:HTB-1™ Price:
Designations:J82Biosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiensMorphology:epithelial Source:Organ: urinary bladder Disease: transitional cell carcinomaPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining thAntigen Expression:HLA A2, Aw32, B5, B12, Cw5; Bloode permits. Please click here for information regarding the specific requirements for shipment to your location.Tumorigenic:YesType ADNA Profile (STR):Amelogenin: X,Y CSF1PO: 10,11 D13S317: 10,12 D16S539: 11,12 D5S818: 12,13 D7S820: 9,11 THO1: 9.3 TPOX: 11,12 vWA: 17,18Cytogenetic Analysis:The cell line is aneuploid human male (XY), with most chromosome counts in the triploid range. However, the chromosome count range is quite broad, extending from hyperdiploid to hexaploid. Normal chromosomes N11 and N20 are under-represented with respect to the other normal chromosomes: altered forms of these two chromosomes are prominent as marker chromosomes. Chromosome N13 tends towards over-representation. Five marker chromosomes are noted: 20q+, 11q+, 8p+, del(1)(q31), 5p+(HSR). One of these, 20q, is identical to marker chromosome M1 of C. O'Toole, et al., Br. J. Cancer 38: 64, 1978. The remainder are not as clearly related to the original marker chromosomes noted by those authors.Isoenzymes:AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1-2 PGM1, 1 PGM3, 2Age:58 yearsGender:male                       J82 細(xì)胞,人膀胱移行細(xì)胞癌細(xì)胞Ethnicity:Caucasian, SwedishComments:Electron microscopic examination did not reveal desmosomes but varying amounts of rough endoplasmic reticulum and prominent microfilaments were observed. Contains the ras (H-ras) oncogene.Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°CSubculturing:Protocol:Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:10 is recommended Medium Renewal: 2 to 3 times per weekPreservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phaseRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003recommended serum:ATCC 30-2020References:21849: O'Toole CHuman bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitroIn: O'Toole CIn vitro models for cancer researchvol. IVBoca Raton, FLCRC Presspp. 103-125.22325: O'Toole C, et al. Ultrastructure, karyology and immunology of a cell line originated from a human transitional-cell carcinoma. Br. J. Cancer 38: 64-76, 1978. PubMed: 68751924381: Fogh J. C*tion, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 57104725065: Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 901248432266: Bender CM, et al. Inhibition of DNA methylation by 5-Aza-2'-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res. 58: 95-101, 1998. PubMed: 9426064




















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
亚洲欧美日韩久久精品| 精品无码成人网站久久久久久| 国产成人精品无码片区在线观看 | 欧美国产亚洲高清| 亚洲人妻中文字幕| 被糙汉被禽哭尿进去h| 精品视频在线观看免费观看| 少妇激情一区二区三区视频| 亚洲国产精品久久久久婷婷软件 | 国产aⅴ夜夜欢一区二区三区| 国产在线一区二区在线视频| 亚洲无码在线精品视频| 在线高清一本av| 99久久综合精品五月天| 国产精品成人影院在线观看| 九一麻豆蜜臀| 狠狠躁天天躁日日躁欧美| 青青爽无码视频在线观看| 国产精品免费一区二区三区四区 | 久久久久噜噜噜亚洲熟女综合| 玩弄人妻少妇500系列网址| 国产免费AV片在线观看播放| 精品久久久一区| 精品一区二区三区东京热| 亚洲欧洲自拍拍偷午夜色无码| 日韩一级黄片| 国产交换配乱婬视频| 美日韩精品无码AV专区久久久 | 国产欧美在线亚洲一区| 中国性生活片| 国产无内肉丝精品视频| 香蕉网| 国产精品麻豆欧美日韩WW| 两个黑人大战嫩白金发美女 | 黑人与少妇| 欧美综合自拍亚洲综合百度| 亚洲小黄片| 久久A级毛片免费观看| 欧美性XXXXX极品| 国产片av国语在线观看手机版| 精品亚洲日韩AⅤ在线观看|