久久久天堂国产精品女人,国产AV国片精品一区二区,伊人久久大香线蕉av影院,日韩av

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2557Panc 05.04細(xì)胞, 人胰腺癌細(xì)胞

Panc 05.04細(xì)胞, 人胰腺癌細(xì)胞

簡要描述:Panc 05.04細(xì)胞, 人胰腺癌細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-2557
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-21
  • 訪  問  量:1366

產(chǎn)品分類

Product Category

相關(guān)文章

Related Articles

詳細(xì)介紹

Panc 05.04細(xì)胞, 人胰腺癌細(xì)胞

ATCC® Number:CRL-2557™    Price:$417.00
Designations:Panc 05.04Depositors:EM JaffeeBiosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Morpholoepithelial Source:gy:Organ: pancreas Disease: adenocarcinomaCellular Products:cytokeratins 7 and 18 [50655]Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.Panc 05.04細(xì)胞, 人胰腺癌細(xì)胞Isolation:Isolation date: May 5, 1995Tumorigenic:YesOncogene:K-ras +Antigen Expression:MHC class I +; MHC class II - [50655] Blood type B; Rh+Age:77 years adultGender:femaleEthnicity:WhiteComments:Panc 05.04 is a pancreatic adenocarcinoma epithelial cell line derived, in 1995, from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma. The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. [50655] The cells have a reported plating efficiency of 100%. [50655]Propagation:ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 20 Units/ml human recombinant insulin, 85%; fetal bovine serum, 15%Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°CSubculturing:Protocol:                    Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.Incubate cultures at 37°C.Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:5 is recommended Medium Renewal: Add media once per week. Fluid change one to two times per week.Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phaseDoubling Time:46 hrsRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001recommended serum:ATCC 30-2020References:50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
第一次人妻| 国语自产偷拍精品视频偷拍图片| 综合色区亚洲熟妇另类| 蜜桃视频一区二区三区在线观看| 欧美亚洲另类丝袜综合网| 在线观看亚洲AV每日更新无码| 亚洲中文字幕在线第二页| 久久久成人网| 五月婷婷色| 精品亚洲一区二区三区四区五区高| 国产日韩av在线| 三级黄视频| 精品中文字幕| 少妇性xxxxxxxxx色孕交| 午夜精品久久久久久久久| 国产91免费| 国产成人午夜高潮毛片| 国产av主播| 国产精品麻豆成人av电影艾秋| 精品| 午夜老湿机| 日韩一级二级三级| 国产成人精品亚洲男人的天堂| 亚洲精品国产精品乱码不卡√香蕉| 久久久福利| 属兔人永远最旺的颜色| 一本au道大尺码高清专区| 久久大伊人| 色婷婷综合久久久中文字幕| 在线色播| 国产熟女一区| 国产性70yerg老太| 龙胜| 久久亚洲国产成人影院| 国产AV熟女一区二区三区| 免费天堂无码人妻成人av电影| 老熟女富婆激情刺激对白 | 最近高清日本免费| 在线播放亚洲第一字幕| 亚洲性猛交xxxx| 精品国产三级a在线观看 |