久久久天堂国产精品女人,国产AV国片精品一区二区,伊人久久大香线蕉av影院,日韩av

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2119HPAC 人胰腺癌細(xì)胞

HPAC 人胰腺癌細(xì)胞

簡(jiǎn)要描述:CRL-2119 HPAC 人胰腺癌細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-2119
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-12
  • 訪  問  量:1807

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2119 HPAC 人胰腺癌細(xì)胞

ATCC® Number: CRL-2119™ Price: $323.00

Designations: HPAC

Depositors: WR Gower

Biosaum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: pancreasfety Level: 1

Shipped: frozen

Medium & Ser

Disease: adenocarcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


CRL-2119 HPAC 人胰腺癌細(xì)胞

Isolation: Isolation date: 1985

Receptors: epidermal growth factor (EGF), expressed

glucocorticoid, expressed

epidermal growth factor (EGF); glucocorticoid

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 13

D13S317: 11

D16S539: 9,10

D5S818: 12

D7S820: 10,12

THO1: 9.3

TPOX: 10,11

vWA: 15,17

Cytogenetic Analysis: modal number = 61

Age: 64 years

Gender: female

Ethnicity: Caucasian

Comments: HPAC is a pancreatic adenocarcinoma epithelial cell line derived in 1985 from a nude mouse xenograft of a primary tumor removed from the head of the pancreas of a woman with moderate to well differentiated pancreatic adenocarcinoma of ductal origin.

HPAC proliferation is stimulated by insulin, insulin-like growth factor I (IGF-I), epidermal growth factor (EGF), and transforming growth factor alpha (TGF alpha).

Cell growth is suppressed by dexamethasone and other glucocorticoids.

HPAC cells are positive for keratin and negative for vimentin and chromogranin A.

Immunohistochemical staining revealed that HPAC cells express the pancreatic ductal epithelium marker DU-PAN-2 as well as antigens recognized by the monoclonal antibodies HMFG1 and AUA1.

Crude HPAC cell exacts contained significant concentrations of tumor-associated antigens CEA, CA 125, and CA 19-9.

In culture, HPAC cells form monolayers of morphologically heterogenous polar epithelial cells.

HPAC is the first reported human pancreatic adenocarcinoma cell line to express a functional glucocorticoid receptor.

Propagation: ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM sodium pyruvate supplemented with 0.002 mg/ml insulin, 0.005 mg/ml transferrin, 40 ng/ml hydrocortisone, 10 ng/ml epidermal growth factor and 5% fetal bovine serum

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended

Medium Renewal: Every 3 to 4 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 41 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

recommended serum:ATCC 30-2020

References: 22987: Gower WR Jr., et al. HPAC, a new human glucocorticoid-sensitive pancreatic ductal adenocarcinoma cell line. In Vitro Cell. Dev. Biol. 30A: 151-161, 1994.



CRL-2119


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
中文字幕精品一区| 亚洲乱码无码永久不卡在线| 欧美乱性| 好爽又高潮了毛片免费下载| 国产亚洲精品a在线看| 精品亚洲成在人线AV无码| 国产精品1区2区| 欧美一二| 小12萝自慰喷水亚洲网站| 成人无码精品一区二区三区| 免费久久99精品国产自在现| 第九色| 777777色狠狠俺88888| 亚洲色无码一区二区三区| 国产成人精品手机在线观看| 尤物精品视频无码福利网| 少妇| 国产最猛性xxxx69交| 成年人天堂com| 777米奇久久最新地址| 国产精品久久久久久久久KTV | 亚色av| 日狠狠| 色婷婷网| 少妇视频网站| 久久久精品影院| 国产大学生粉嫩无套流白浆| 亚洲国产精品国自产拍电影| 一本之道中文日本高清| 国产精品美女久久久浪潮av| 国产精品久久久久AV福利动漫| 人妻美妇疯狂迎合系列视频| 日日操av| 日本一码二码三码| 国产一区二区三区国产一区| 综合欧美日韩国产成人| 中文字幕精品亚洲无线码二区| 特大巨黑吊性xxxx| 久久久精品波多野结衣| 免费无码又爽又刺激毛片| 日韩国产精品亚洲а∨天堂免|