久久久天堂国产精品女人,国产AV国片精品一区二区,伊人久久大香线蕉av影院,日韩av

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>小鼠腫瘤細(xì)胞、癌細(xì)胞>CRL-1720F9 小鼠畸胎瘤細(xì)胞

F9 小鼠畸胎瘤細(xì)胞

簡(jiǎn)要描述:CRL-1720 F9 小鼠畸胎瘤細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種原代細(xì)胞、細(xì)胞系。細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-1720
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問(wèn)  量:1962

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1720 F9 小鼠畸胎瘤細(xì)胞


ATCC® Number:  CRL-1720™

Designations:  F9

Depositors:   S Strickland

Biosafety Level: 1

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Mus musculus (mouse)

Morphology: epithelial

CRL-1720 F9 小鼠畸胎瘤細(xì)胞

Source: Organ: testis

Strain: 129

Disease: embryonal carcinoma; testicular teratoma

Cellular Products: plasminogen activator; laminin; type IV collagen

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applications: transfection host (Roche FuGENE® Transfection Reagents)

Age:  embryo

Comments: F9 cells can be stimulated to differentiate into parietal endoderm in the presence of retinoic acid and dibutyryl cyclic AMP (cAMP).

Differentiating cells synthesize plasminogen activator, laminin and type IV collagen.

cAMP is active only on cells that have been treated with retinoic acid.

The cells maintain three copies of the beta 1 integrin gene.

Tested and found negative for ectromelia virus (mousepox).

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing:  Protocol: NOTE: culture vessels must be coated with 0.1% gelatin prior to use.To do so, cover the surface of the vessel with 0.1% gelatin (Difco) in sterile distilled water for 2 hours at 4C, then wash three times with sterile distilled water. Treated flasks and dishes can be stored at room temperature.

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new coated culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:10 is recommended

Medium Renewal: Every 2 to 3 days

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 1160: Strickland S, et al. Hormonal induction of differentiation in teratocarcinoma stem cells: generation of parietal endoderm by retinoic acid and dibutyryl cAMP. Cell 21: 347-355, 1980. PubMed: 6250719

1161: Strickland S, Mahdavi V. The induction of differentiation in teratocarcinoma stem cells by retinoic acid. Cell 15: 393-403, 1978. PubMed: 214238

23426: Stephens LE, et al. Targeted deletion of beta 1 integrins in F9 embryonal carcinoma cells affects morphological differentiation but not tissue-specific gene expression. J. Cell Biol. 123: 1607-1620, 1993. PubMed: 7504677

26151: Berstine EG, et al. Alkaline phosphatase activity in mouse teratoma. Proc. Natl. Acad. Sci. USA 70: 3899-3903, 1973. PubMed: 4521215

32547: Jang SI, et al. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J. Biol. Chem. 271: 24105-24114, 1996. PubMed: 8798649



CRL-1720


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
中文字幕一区二区人妻| 波多野结衣中文字幕久久| 日韩精品中文字幕一区二区三区| 中国女人内谢69XXXX视频| 影音先锋每日av色资源站| 精品一区二区免费视频蜜桃网| 99rαv少妇裸体高潮av| 久久久xxx| 四虎国产精品永久免费网址| 77777亚洲午夜久久多喷| 色欲色香天天天综合VVV| 91久久| 放荡肉欲狂浪妇| bl小说网| 无码AV波多野结衣久久| 亚洲AV日韩AV天堂一区二区三区| 97久久精品亚洲中文字幕无码| 久久精品国产日本波多野结衣| 亚洲AV成人无码一区在线观看| 亚洲精品天堂成人片AV在线播放| 久久久久亚洲精品无码系列| av无码精品一区二区三区三级| 女优色图| 窝窝午夜看片| 精品国产一区二区三区久久久蜜月| 国产浪潮AV性色四虎| 亚洲av不卡无码国产| 天堂MV在线MV免费MV香蕉| 少妇人妻88久久中文字幕| 国产成人高清在线播放| 美女扒开奶罩露出奶头视频网站| 亚洲www啪成人一区二区| 亚洲国产精品成人综合久久久久久久| 日韩一区中文字幕| 亚洲AV永久无码国产精品久久| 好男人日本社区www| 最新永久免费av无码网站| 国产呻吟久久久久久久92| 好男人资源在线www免费| 野花香日本大全免费观看| 丁香五月网久久综合|