久久久天堂国产精品女人,国产AV国片精品一区二区,伊人久久大香线蕉av影院,日韩av

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>大鼠正常細(xì)胞>CRL-1444A7r5 大鼠胸大動脈平滑肌細(xì)胞

A7r5 大鼠胸大動脈平滑肌細(xì)胞

簡要描述:CRL-1444 A7r5 大鼠胸大動脈平滑肌細(xì)胞, ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號:CRL-1444
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-14
  • 訪  問  量:2276

產(chǎn)品分類

Product Category

相關(guān)文章

Related Articles

詳細(xì)介紹

CRL-1444 A7r5 大鼠胸大動脈平滑肌細(xì)胞, ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件

CRL-1444 A7r5 大鼠胸大動脈平滑肌細(xì)胞的詳細(xì)介紹


ATCC® Number:CRL-1444™  Price:$275.00
Designations:A7r5

Depositors:W Carlisle

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Rattus norvegicus (rat)

Morphology:fibroblast

Source:Organ: aorta, thoracic
Strain: DB1X
Tissue: smooth muscle


Cellular Products:myokinase; creatine phosphokinase (muscle isoenzyme); myosin

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-1444

Applications:transfection host (Roche FuGENE® Transfection Reagents

technology from amaxa)



Age:embryo

Comments:The cells exhibit an increase in activity of the enzymes myokinase and creatine phosphokinase (CPK) as the culture reaches stationary phase.

Muscle type CPK is synthesized after cell division has ceased.



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days


Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020



References:1061: Kimes BW, Brandt BL. Characterization of two putative smooth muscle cell lines from rat thoracic aorta. Exp. Cell Res. 98: 349-366, 1976. PubMed: 943301

CRL-1444




















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
操丝袜人妻| 国产精品久久毛片| 高清国产AV一区二区三区| 欧美人妖hdvideo| 女人爽到高潮视频免费直播| 泰宁县| 免费视频91| 欧美牲交a免费| 久久日韩精品一区二区五区| 国产成人AV一区二区三区不卡| 亚洲大尺度无码无码专线一区| 中文字字幕一区二区三区四区五区| 国产乱码精品一区二区三区四川人| 九九热在线精品视频| 777奇米四色成人影视色区| 狠狠色丁香婷婷久久综合| japonese老熟女老熟妇| 亚洲熟妇AV一区二区三区| 午夜精品久久久久久久久久久久| 亚洲日韩国产一区二区三区在线 | 久久精品无码一级二级| 国产在线观看免费人成视频| 国产成人综合久久亚洲精品| 久久99精品久久久久久婷婷 | 久久久久久综合岛国免费观看| 中文精品无码中文字幕无码专区 | 亚洲制服丝袜av一区二区三区| 丁香综合激情| 国产午夜亚洲精品理论片不卡| 亚洲高清国产拍精品熟女| 国模杨依粉嫩蝴蝶150p| 国产真人无遮挡作爱免费视频| 精品国产三级AV一区二区三区| 无码人妻丰满熟妇啪啪7774| 91久久国产综合久久91雪峰| 放荡的美妇在线播放| mm131美女图片尤物写真丝袜| 99久久精品国产毛片| 大香蕉99| 在线播放国产高潮流白浆视频| 国产欧美日韩亚洲一区二区三区|